KMID : 0606920130210030216
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Biomolecules & Therapeutics 2013 Volume.21 No. 3 p.216 ~ p.221
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Aromadendrin Inhibits Lipopolysaccharide-Induced Nuclear Translocation of NF-¥êB and Phosphorylation of JNK in RAW 264.7 Macrophage Cells
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Lee Jae-Won
Kim Nam-Ho Kim Ji-Young Park Jun-Ho Shin Seung-Yeon Kwon Yong-Soo Lee Hee-Jae Kim Sung-Soo Chun Wan-Joo
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Abstract
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Aromadendrin, a flavonol, has been reported to possess a variety of pharmacological activities such as anti-inflammatory, antioxidant, and anti-diabetic properties. However, the underlying mechanism by which aromadendrin exerts its biological activity has not been extensively demonstrated. The objective of this study is to elucidate the anti-inflammatory mechanism of aromadedrin in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Aromadendrin significantly suppressed LPS-induced excessive production of pro-inflammatory mediators such as nitric oxide (NO) and PGE2. In accordance, aromadendrin attenuated LPSinduced overexpression iNOS and COX-2. In addition, aromadendrin significantly suppressed LPS-induced degradation of I¥êB, which sequesters NF-¥êB in cytoplasm, consequently inhibiting the nuclear translocation of pro-inflammatory transcription factor NF- ¥êB. To elucidate the underlying signaling mechanism of anti-inflammatory activity of aromadendrin, MAPK signaling pathway was examined. Aromadendrin significantly attenuated LPS-induced activation of JNK, but not ERK and p38, in a concentration-dependent manner. Taken together, the present study clearly demonstrates that aromadendrin exhibits anti-inflammatory activity through the suppression of nuclear translocation of NF-¥êB and phosphorylation of JNK in LPS-stimulated RAW 264.7 macrophage cells.
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KEYWORD
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Aromadendrin, COX-2, iNOS, JNK, Lipopolysaccharide, NF-¥êB, RAW 264.7 cells
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